Published: 2021-09-27

Prevalence and antibiotic susceptibility pattern of Klebsiella species isolated from various clinical samples in a tertiary care hospital Coimbatore

Vijayashree V., Saikeerthana D., Prabha P.


Background: The genus Klebsiella of Enterobacteriaceae family is ubiquitous in nature. They cause many nosocomial infections like pneumonia, urinary tract infections, wound infections, bacteremia and septicemia. Multidrug resistance is seen in Klebsiella which serves as the most common cause of increased morbidity and mortality. This study reveals the prevalence and antibiotic sensitivity pattern of Klebsiella species from various clinical samples. The primary objectives are as follows: To isolate and characterize Klebsiella species from various clinical samples. To study the antibiotic susceptibility pattern of Klebsiella isolates.

Methods: This prospective study was conducted in our tertiary care hospital during the period from August 2019 to October 2019. A total of 3521 samples were tested during this period. The samples include blood, sputum, urine, and pus.

Results: Out of the total samples tested,1106 samples were showing the growth of the organisms. Among this,351 were identified as Klebsiella species and the highest rate of isolation of Klebsiella species is from the sputum sample and also the same was reported high in medical wards. The study also shows that the isolation of Klebsiella species shows male preponderance when compared to females. The antibiotic sensitivity pattern was done by Kirby-Bauer's disc diffusion method and the sensitivity was noted to be higher to amikacin and ciprofloxacin.

Conclusions: Thus, this study shows the prevalence rate of Klebsiella species and sensitivity pattern of Klebsiella, which may help select appropriate antibiotics and prevent overuse and misuse of antibiotics.


Klebsiella, Prevalence, Antibiotic sensitivity pattern

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Winn W, Allen S, Janda W, Koneman E, Procop G, Schreckenberger P et al. The Enterobacteriaceae. Koneman’s Color Atlas and Textbook of Diagnostic Microbiology, Lippincott Williams and Wilkins. 6th Edition. New York. 2006.

Forbes BA, Sahim DF, Weissfeld AS. Bailey & Scott’s Diagnostic Microbiology. St. Louis, Mo M. 12th Edition. Elsevier Mosby. 2007.

Ndiba PM. Phenotypic detection and susceptibility pattern for the detection of extended spectrum beta lactamases producing Klebsiella pneumonia isolates in Nairobi, Kenya. Open journal of Medical Microbiology. 2013;3:91-4.

Podschun R, Ullmann U. Klebsiella spp. as nosocomial pathogens: epidemiology, taxonomy, typing methods and pathogenicity factors. Clinical microbiology reviews; 1998;11:589-603.

Bennett JE, Dolin R, Blaser MJ. Mandell, Douglas, and Bennett's principles and practice of infectious diseases. 6th Edition. Elsevier Inc. 2014. 2578-79.

Murray PR, Holmes B, Aucken HM. In: Topley & Wilson’s Microbiology and microbial infections. 10th edition. Salisbury UK, Edward Arnold Ltd. 2005.

Mackie RT, McCartney. Practical Medical Microbiology, JG Collee, JP Duguid, AG Fraser, BP Marmion (Eds.). Churchill Livingston. 14th edition. Chichester. 1989:369.

CLSI Performance Standards for antimicrobial disk susceptibility tests, 10th edition, CLSI standard M 02, Wayne PA. 2006.

Ravichitra KN, Prakash PH, Subbarayudu S, Rao US. Isolation and antibiotic sensitivity of Klebsiella pneumoniae from pus, sputum and urine samples. Int J Curr Microbiol App Sci. 2014;3:115-19.

Alves MS, da Silva Dias RC, de Castro AC, Riley LW, Moreira BM. Identification of clinical isolates of indole-positive and indole-negative Klebsiella spp. Journal of clinical microbiology 2006;44:3640-46.

Akter J, Chowdhury AM, Al Forkan M. Study on prevalence and antibiotic resistance pattern of Klebsiella isolated from clinical samples in South East Region of Bangladesh. American Journal of Drug Discovery and Development. 2014;4:73-9.

Thosar MG, Kamble VA. Occurrence of ESBL producing Klebiella species in clinical samples. Indian Journal of applied research. 2014;4:408-10.

Sarathbabu R, Ramani TV, Rao KB, Panda S. Antibiotic susceptibility pattern of Klebsiella pneumoniae isolated from sputum, urine and pus samples. IOSR J Pharm Biol Sci. 2012;1:04-09.

Sikarwar AS, Batra HV. Prevalence of antimicrobial drug resistance of Klebsiella pneumoniae in India. International Journal of Bioscience, Biochemistry and Bioinformatics. 2011;1:211-5.

Pavani G. Drug susceptibility pattern of Klebsiella and Citrobacter infections in India. J Microbiol Biotech Res. 2012;2:619-20.

Kumar AR, Kalpana S. Prevalance and antimicrobial susceptibility pattern of Klebsiella pneumonia causing UTI and issues related to the rational selection of antimicrobials. Scholars Journal of Applied Medical sciences. 2013;1:395-99.

Rajeevan S, Ahmad SM, Jasmin PT. Study of prevalence and antimicrobial susceptibility pattern in blood isolates from a tertiary care hospital in North Kerala, India. Int J Curr Microbiol Appl Sci. 2014;3:655-62.