DOI: http://dx.doi.org/10.18203/2394-6040.ijcmph20211768

Correlation of COVID-19 antigen test and CBNAAT results at a single tertiary care hospital in North India: an experience

Surbhi Gupta, Anju Shukla, Poonam Singh, Areena H. Siddiqui

Abstract


Background: Nucleic acid amplification test (NAAT) is considered gold standard in the molecular diagnosis of CoV-2 infection but since it is costly, labor intensive and needs technical expertise, rapid chromatographic immunoassay for the qualitative detection of specific antigens to SARS CoV-2 have been devised. Objectives of this study was to compare the results of Antigen test and NAAT for CoV-2 infection carried out during the months of July and August 2020 by single tertiary care hospital in Lucknow, Uttar Pradesh and to determine the utility of rapid antigen test in the SARS CoV-2 diagnosis.

Methods: All the patients who came to our hospital seeking admission during July 2020 and August 2020 were included in the study. A total of 1000 patients were included in this study.

Results: Out of a total 1000 cases which were included in the study, 769 cases (76.9%) were found to be SARS CoV-2 negative by both antigen and CBNAAT, 100 cases (10.0%) were SARS CoV-2 positive by both antigen and CBNAAT tests. But in 131 cases (13.1%), antigen was not able to pick up the disease. It was also found that the Cycle Threshold (Ct) value for the discordant group was higher (Mean E= 28, Mean N2=33) when compared to the group where antigen was positive.

Conclusions: The present study establishes the role of rapid antigen tests in contributing to the quick, point of care diagnosis of SARS CoV-2. These assays are safe, simple, and fast and can be used in local clinics and hospitals. These tests are very important for real-time patient management and infection control decision.


Keywords


COVID-19, CBNAAT, Rapid Antigen Test, SARS-CoV-2

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References


Carter L. Assay techniques and test development for COVID-19 Diagnosis. ACS Central Science2020. Available at https:// doi. org/ 10.1021/acscentsci.0c00501. Accessed on 12 December 2020.

Tahamtan A, Abdollah A. Real-Time RT-PCR in COVID-19 detection: issues affecting the results. Expert Review of Molecular Diagnostic. 2020;20(5);453-4.

Mertens P, De Vos N, Martiny D, Jassoy C, Mirazimi A, Cuypers L, et al. Development and potential usefulness of the COVID-19 Ag respi-strip diagnostic assay in a pandemic context. Front Med. 2020;7(225):10-33.

Poole S. How are rapid diagnostic tests for infectious diseases used in clinical practice: a global survey by the International Society of Antimicrobial Chemotherapy (ISAC). Eur J Clin Microbiol Infect Dis. 2020;8:73-9.

Rubin EJ, Baden LR, Morrissey S. Audio interview: new research on possible treatments for Covid-19. N Engl J Med. 2020;382:e30.

Ong DSJ, Man SJ, Lindeboom SA, Koeleman JGM. Comparison of diagnostic accuracies of rapid serological tests and ELISA to molecular diagnostics in patients with suspected coronavirus disease 2019 presenting to the hospital. Clin Microbiol Infect. 2020;26(8):33-9.

Corman VM. Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR. Euro Surveill. 2020;25(3):33-9.

Lu R. Genomic characterisation and epidemiology of 2019 novel coronavirus: implications for virus origins and receptor binding. Lancet. 2020;30:30251-8.

Khairat SM, Guindy N, Motaleb EA, Soliman NA. Evaluation of two rapid antigen tests for detection of SARS-CoV-2 virus. Int J Microbiol Biotech. 2020;5(3):131-4.

Hirotsu S. Comparison of automated SARS-CoV-2 antigen test for COVID-19 infection with quantitative RT-PCR using 313 nasopharyngeal swabs, including from seven serially followed patients. Int J Inf Ds. 2020;99:397-402.

Scohy A, Anantharajah A, Bodéus M, Kabamba-Mukadi B, Verroken A, Villalobos H. Low performance of rapid antigen detection test as frontline testing for COVID-19 diagnosis. J Clin Virol. 2020;129:1044-55.