Test the effectiveness of antibacterial effect of the skin of the pomegranate fruit (Punica granatum) extract against the growth of Escherichia coli in vitro

Niken ., Eliza Arman, Rahmi Novita Yusuf, Honesty Diana Morika


Background: Pomegranate (Punica granatum) is one of the traditional medicines that contains antibacterial compounds that are effective against bacterial growth. Its chemical content includes saponins, tannins and flavonoids. Escherichia coli is a gram-negative bacterium which is a normal flora germ found in the human large intestine. These bacteria are pathogenic when they are outside the intestine and produce enterotoxins in epithelial cells which cause diarrhea. The purpose of this study was to determine the antibacterial effect of pomegranate skin extract on the growth of Escherichia coli.

Methods: This study used an experimental design study with a complete randomized design study divided into 5 groups namely groups 1 (15 ug/ml), 2 (20 ug/ml), 3 (25 ug/ml), positive control (ciprofloxatin), negative control. Making pomegranate peel extract was done by maceration method then rotary, after that the effectiveness of pomegranate extract extracted by the diffusion method was tested using Anova one-way test.

Results: The results showed that the extract of pomegranate peel showed that it was able to inhibit the growth of Escherichia coli with a ratio of constants (15 ug/ml, 20 ug/ml, 25 ug/ml with inhibition diameters of 6.7 mm, 6.7 mm, 6, 7 mm, while for positive control with ciprofloxatin showed bacterial resistance to antibiotics.

Conclusions: Statistically, pomegranate skin extract has an antibacterial power which is meaningful with p 0.005. Pomegranate rind extract has antibacterial activity against Escherichia coli medium because inhibition zone is 5-10 mm.


Antibacterial, Escherichia coli, Pomegranate

Full Text:



Al-Zoreky NS. Antimicrobial activity of pomegranate (Punica granatum L.) the skin of the fruit. The J Food Microbiol. 2009;14 (3):123-67.

Amalia P. Extract formulations skin with pomegranates and leaves dewandaru as sediment antipsikeri and the use of zeolite Untukmenaga Formula 1stabilitas. Thesis. Institut Pertanian Bogor; 2012:142-164.

Asmardi, Arifian, Roza rh, Fitmawati. Antivitas Antibacterial Extract D'aun Cyclea Barbata (l.) Miers against Bakcheri Escherichia coli and Salella tipila. Jom Faculty Sci. 2014;1(2):1-9.

Aziz, Tamzil, Febrizky S, Mario AD. The influence of Solvent type on percent yieldalkaloid of the bay leaf of India (Murraya koenigii). Tech Chemist. 2014:2(20):1-6.

Cushnie TT, Lamb AJ. Antimicrobial activity of flavonoids. Int J Antimicro Agent. 2005;26(5):343-56.

Davis WW, Stout TR. Disc plate method of microbiological antibiotic assay: II. Novel procedure offering improved accuracy. Appl Environ Microbiol. 1971;22(4):666-70.

Eve LC. Studies Kompasi Inactivation of Escherichia coli and Changes in the Physical Properties of the Pasde Paseu Pasteu Sappi Seggaun Methods and Metanas and Denganajutri List. J Agricultural Technol. 2011;12(1):31-9.

Hendra R, Ahmad S, Sukari District A, Shukor MY, Oskoueian E. Flavonoids and analysis of the activity of the antimicrobial properties of various parts of Phaleria Macrocarpa (Scheff.) Fruit Boerl. Int J Mol Sci. 2011;12(5):342-31.

Ismarani. The potential Snyawa tannin in supporting the production of environmentally friendly. CEEFARS: J Agribu Reg Develop. 2012;3(2):69-76.

Jaktaji RP, Ebadi R, Karimi M. Study the substances of the organic solvent tolenrance and create antitibiotik resistance property of E. coli gyrA Mutants. J Pharma Res Iranian. 2012;11(2):595-600.

Jannata H, Gunadi A, Ermawati T. The power of antibacterial extract of the skin manalagi apple (Malus sylvestris mill.) against the growth of streptococcus mutans. E-J Herit Health. 2014;2(1):23-8.

Jawetz E, Melnick J, Adelberg EA. Microbiology medical Edition 1. Translated by microbiology section, faculty of kedokteranitas Airlangga. Salemba Medika. 1996:308-401.

Katzung BG, Masters SB, Trevor AJ. Pharmacologist basic and clinic, volume 2, 12th edition, Editor Language Indonesia Ricky Soeharsono et al, Publisher of Medical Books EGC, Jakarta; 2007:98-104.

Kholisa, Purwanto, Hernawati S. The potential extract of red pomegranate (Punica granatum Linn) against pen jumuni strapptoccus mutansus (the potential of the fruit extract pomat pomic pany Linn) on the number of fractional colonies stretokptocus). E-J Herit Health. 2018;6(2):90-125.

Lenny, Suvia. Compounds flavonoids, fenilpropanoida, and alkaloids. Not published. scientific works. Department of chemistry, faculty of science, University of Sumatra. Repository USU. 2006:145-149.

Madhawati R. The beautiful pomegranate (Punica granatum) With a Million Benefits antioxidant as Bazhatif Natural Healthy and Youthful. Univer Malang. 2012:87-98

Madigan, David MT, Clark P, David S, John MM. Brock microbiology of microorganisms. Benjamin Cumm Publis. 2005:47-69.

Martiyah. Resistance test staphylocococcus aureusterahadapotik, antibiotics amoxillin, tetracycline and propolis. Journal for you of Nature Envir Con. 2019;12(6):307-408.

Nauli RR. The influence of the administration of the extract gravy fruit delima putih (punnatum linn) danokonazol 2% terrahapertumbuhan candicanscara inro padro padaidi vulvica granovaginalis. Thesis. Univer Diponegoro; 2010:99-106.

Pereira CL, Higgis NP, Krezer KN, Morrison A, Brown PO, Sugionom A, et al. The structure and activity of Escherichia coli DNA chrase. cold spring harb, symp. Quant Biol. 2007;43(1):41-52.

Prihantoro TR, Sumerno. Antibacterial effect ekstrakuk skin fruit of pomegranate (punica granatum) against shigentriae in vitro. J Med Brawij. 2006;12(3):39-58.

Putra IM. Antibacterial activity test of ethanol extract of soursop (annonae muricata L.) leaves with the Diffusion Method for Discs to Escherichia coli. J Ilmi Med. 2015;1(1):15-9.

Son, I. Amanda, Erly, and Machdawaty Masri. Test the Antibacterial Effect of Ethanol Extract of skin Stem Greetings {Syzigium polyantum (Wight) Walmap Staphylap Staphylococci coli in invitro. Med J Lombok. 2015;4(2):497-501.

Sap W. Profile of the plasmid and the pattern of resistance of the germs E. coli in patients with UTI in Rsudam Provampir Lampung. Thesis. FMIPA UNILA; 2015:35-56

Suryati N, Bahar E, Ilmiawati. Test the effectiveness of antibacterial ekstrakaloe vera abdahadap the growth of escherichia coli in in vitro. J Health Lombok. 2017;6(3):65-78.

Tjitrosepomo, G. Taxonomy Torumbuhan. Gadjah Mada University Press, Yogyakarta, first edition; 1993;4(3):116-26.

Todar. Text book online of Bacteriologi, 2008. Available at: Accessed on 17th July 2019.

The World Health Organization. Use of the drug in primary care and developing countries; 2012: 141-164.