Antimicrobial susceptibility profile of Vibrio cholerae strains isolated at a tertiary care medical centre in New Delhi, India
DOI:
https://doi.org/10.18203/2394-6040.ijcmph20170775Keywords:
Antibiogram, Cholera, DiarrheaAbstract
Background: Vibrio cholerae is a common cause of acute diarrhea in India. Extensive and injudicious use of antimicrobials has led to the emergence of V. cholerae strains that are resistant to several antibiotics. The present study was conducted to document the antibiogram of V. cholerae strains isolated at an urban health centre in New Delhi. The present study was a retrospective, record-based analysis conducted from January to December 2015, at the Department of Microbiology of a tertiary care teaching institute.
Methods: Stool samples received in the laboratory were processed as per standard microbiological techniques and all the V. cholerae strains isolated were subjected to antimicrobial susceptibility testing as described in the Clinical and Laboratory Standards Institute (CLSI) guidelines. Data was analyzed using the Epi info software, version 3.5.3, and results depicted in the form of descriptive statistics.
Results: Vibrio cholerae was isolated in 70 of the 2340 stool samples processed during the study period. Serogrouping revealed that 66 (94.3%) of the isolates were V. cholerae O1 serotype Ogawa, while non-O1 non-O139 serogroups constituted 4 (5.7%) isolates. The strains exhibited resistance to nalidixic acid (94.3%), ampicillin (92.9 %), ciprofloxacin (55.7%), cefotaxime (41.4%), gentamicin (22.9 %) and amikacin (7.1 %).
Conclusions: Our study highlights the significance of regular surveillance of antimicrobial resistance profile of V. cholerae strains for proper management of cholera.
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References
Das S, Choudhry S, Saha R, Ramachandran VG, Kaur K, Sarkar BL. Emergence of multiple drug resistance Vibrio cholerae O1 in East Delhi. J Infect Dev Ctries. 2011;5(4):294-8.
Das S, Saha R, Kaur IR. Trend of antibiotic resistance of Vibrio cholerae strains from East Delhi. Indian J Med Res. 2008;127(5):478-82.
Sharma NC, Mandal PK, Dhillon R, Jain M. Changing profile of Vibrio cholerae O1, O139 in Delhi & its periphery (2003-2005). Indian J Med Res. 2007;125(5):633-40.
Ramamurthy T, Sharma NC. Cholera outbreaks in India. Curr Top Microbiol Immunol. 2014;379:49-85.
Roychowdhury A, Pan A, Dutta D, Mukhopadhyay AK, Ramamurthy T, Nandy RK, et al. Emergence of tetracycline-resistant Vibrio cholerae O1 serotype Inaba, in Kolkata, India. Jpn J Infect Dis. 2008;61(2):128-9.
Thornsberry C. Trends in antimicrobial resistance among today's bacterial pathogens. Pharmacotherapy. 1995;15(1 Pt 2):3S-8S.
Kitaoka M, Miyata ST, Unterweger D, Pukatzki S. Antibiotic resistance mechanisms of Vibrio cholerae. J Med Microbiol. 2011;60(Pt 4):397-407.
Garg P, Chakraborty S, Basu I, Datta S, Rajendran K, Bhattacharya T, et al. Expanding multiple antibiotic resistance among clinical strains of Vibrio cholerae isolated from 1992–7 in Calcutta, India. Epidemiol Infect. 2000;124(3):393-9.
Miyazato T, Tamaki Y, Sithivong N, Phantouamath B, Insisiengmay S, Higa N, et al. Antibiotic susceptibility and its genetic analysis of Vibrio cholerae non-O1, non-O139 from environmental sources in Lao People's Democratic Republic. Trop Med Health. 2004;32:245-8.
Porter IA, Duguid JP. Vibrio: Aeromonas: Pleisomonas: Spirritum. In: Collee JG, Duguid JP, Fraser AG, Marmion BP, editors. Mackie and McCartney practical medical microbiology, 13th ed. Edinburgh: Churchill Livingstone; 1989 p. 505-24.
Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing; twenty second informational supplement, 32:M100-S22. 2012.
Kumar A, Oberoi A. Vibrio isolates from cases of acute diarrhea and their antibiotic susceptibility pattern in a tertiary care hospital of Punjab. Chrismed J Health Res. 2014;1(4):254-7.
Hofer E, Quintaes BR, dos Reis EM, Rodrigues Ddos P, Seki LM, Feitosa IS, et al. The emergence of multiple antimicrobial resistance in Vibrio cholerae isolated from gastroenteritis patients in Ceará, Brazil. Rev Soc Bras Med Trop. 1999;32(2):151-6.
Taneja N, Kaur J, Sharma K, Singh M, Kalra JK, Sharma NM, et al. A recent outbreak of cholera due to Vibrio cholerae O1 Ogawa in & around Chandigarh, North India. Indian J Med Res. 2003;117:243-6.
Chandrasekhar MR, Krishna BV, Patil AB. Changing characteristics of Vibrio cholerae: emergence of multidrug resistance and non-O1, non-O139 serogroups. Southeast Asian J Trop Med Public Health. 2008;39(6):1092-7.
Mukhopadhyay AK, Garg S, Nair GB, Kar S, Ghosh RK, Pajni S, et al. Biotype traits and antibiotic susceptibility of Vibrio cholerae serogroup O1 before, during and after the emergence of the O139 serogroup. Epidemiol Infect. 1995;115(3):427-34.
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