Enhancing blood safety: the utility of NS-1 antigen capture ELISA for detecting acute dengue infections among blood donors during peak transmission periods and the potential to curtail dengue spread

Authors

  • Preethika Ravi Mysore Medical College and Research Institute, Mysore, Karnataka, India
  • Deepa S. Mysore Medical College and Research Institute, Mysore, Karnataka, India
  • Manjunath B. S. K. R. Hospital Blood bank, Mysore, Karnataka, India

DOI:

https://doi.org/10.18203/2394-6040.ijcmph20233772

Keywords:

Dengue fever, Transfusion transmission, Screening

Abstract

Background: Dengue fever is a viral disease transmitted by mosquitoes and affects millions of people worldwide. It can also be transmitted through blood transfusions, particularly during peak transmission periods.

Methods: This was a cross sectional study which was conducted over a two-month period (June 2022 to July 2022) at a tertiary hospital blood bank. A total of 180 nonrepetitive serum samples were collected from voluntary blood donors and analyzed using the dengue NS-1 Ag Microlisa ELISA kit.

Results: Among the 180 samples two samples tested positive for the dengue virus NS-1 antigen using the NS-1 antigen capture ELISA, indicating a prevalence of acute dengue virus infection among blood donors during the peak transmission period of 1.2% (2/180).

Conclusions: The use of NS-1 antigen testing was shown to be useful for dengue virus detection. Routine screening of blood donors for dengue virus is not universally implemented in many countries, partly due to the lack of sensitive and specific screening tests. However, the use of serological tests, such as ELISA for dengue antibodies or NS-1 antigen, can effectively detect dengue virus in blood donors. Interventions made during peak transmission periods can help in curtailing the spread of dengue infections.

References

World Health Organization. Dengue and severe dengue WHO; 2021. Available at: https://www.who.int/news-room/fact-sheets/detail/dengue-and-severe-dengue. Accessed on 25 June 2023.

Sabino EC, Loureiro P, Lopes ME, Capuani L, McClure C, Chowdhury D et al. Transfusion-transmitted dengue and associated clinical symptoms during the 2012 epidemic in Brazil. J Infect Dis. 2016;213(5):694-702.

Chuang VW, Wong TY, Leung YH, Ma ES, Law YL, Tsang OT et al. Review of dengue fever cases in Hong Kong during 1998 to 2005. Hong Kong Med J. 2008;14(3):170.

Chaterji S, Allen Jr JC, Chow A, Leo YS, Ooi EE. Evaluation of the NS1 rapid test and the WHO dengue classification schemes for use as bedside diagnosis of acute dengue fever in adults. Am J Trop Med Hyg. 2011;84(2):224.

Dussart P, Petit L, Labeau B, Bremand L, Leduc A, Moua D et al. Evaluation of two new commercial tests for the diagnosis of acute dengue virus infection using NS1 antigen detection in human serum. PLoS Neglect Trop Dis. 2008;2(8):e280.

World Health Organization. WHO guidelines on assessing donor suitability for blood donation. WHO. 2009. Available at: https://www.who.int/bloodsafety/ assessment/bts_guidelines.pdf. Accessed on 25 June 2023.

Dengue situation in India. Available at: https://ncvbdc.mohfw.gov.in/index4.php?lang=1&level=0&linkid=431&lid=3715. Accessed on 25 June 2023.

National Center for Vector Borne Diseases Control. Available at: https://ncvbdc.mohfw.gov.in/index4. php?lang=1&level=0&linkid=432&lid=3714. Accessed on 25 June 2023.

Stramer SL, Linnen JM, Carrick JM, Foster GA, Krysztof DE, Zou S et al. Dengue viremia in blood donors identified by RNA and detection of dengue transfusion transmission during the 2007 dengue outbreak in Puerto Rico. Transfusion. 2012;52(8):1657-66.

World Health Organization Dengue and Severe Dengue. Media Centre Fact Sheet. WHO, 2013. Available at: https://www.who.int/mediacentre/ factsheets/fs117/en/. Accessed on 25 June 2023.

Dengue and Severe Dengue. World Health Organization. Available at: http://www.who.int/news-room/fact-sheets/detail/ dengue-and-severe-dengue. Accessed on 25 June 2023.

Tang KF, Ooi EE. Diagnosis of dengue: an update. Expert Rev Anti Infect Ther. 20121;10(8):895-907.

Clinical Guidance. Dengue. CDC. Available at: https://www.cdc.gov/dengue/clinicallab/clinical.html. Accessed on 25 June 2023.

Mangwana S. Dengue viremia in blood donors in Northern India: Challenges of emerging dengue outbreaks to blood transfusion safety. Asian J Transfusion Sc. 2015;9(2):177.

Guzman MG, Gubler DJ, Izquierdo A, Martinez E, Halstead SB. Dengue infection. Nature Rev Dis Prim. 2016;2(1):1-25.

Raj AR, Shashindran N, Shenoy V, Kumar A. Dengue seropositivity among blood donors in a tertiary hospital in Kerala, Southern India. Ann Afr Med. 2022;21(1):39.

Ashshi MA, Alghamdi S, El-Shemi AG, Almdani S, Refaat B, Mohamed AM et al. Seroprevalence of asymptomatic dengue virus infection and its antibodies among healthy/eligible Saudi blood donors: findings from holy Makkah city. Virology: Research and Treatment. 2017;8:1178122X17691261.

Dean CL, Wade J, Roback JD. Transfusion-transmitted infections: an update on product screening, diagnostic techniques, and the path ahead. J Clin Microbiol. 2018;56(7):10-128.

Seltsam A. Pathogen inactivation of cellular blood products-an additional safety layer in transfusion medicine. Frontiers Med. 2017;4:219.

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Published

2023-11-30

How to Cite

Ravi, P., S., D., & B. S., M. (2023). Enhancing blood safety: the utility of NS-1 antigen capture ELISA for detecting acute dengue infections among blood donors during peak transmission periods and the potential to curtail dengue spread. International Journal Of Community Medicine And Public Health, 10(12), 4739–4742. https://doi.org/10.18203/2394-6040.ijcmph20233772

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Section

Original Research Articles