Effect of supplementation of Purslane-Portulaca oleracea in hypercholesterolemic subjects
Keywords:Medicinal, Food adjunct, Hypercholesterolemic, Supplementation, Cholesterol and its fractions
Background: Portulaca oleracea (Purslane) is a medicinal plant containing diverse phyto constituents grown all over the world. Several studies have reported its pharmacological effects like, antihypertensive, anti-inflammatory antioxidant, hypoglycemic, hypocholesterolemic, hypotriglyceridemia neuroprotective effects.
Methods: Dried Portulaca oleraca leaves were incorporated into spice powder/ karampodi a south India food adjunct which was used for supplementation studies for 90 days (3 months) among hyper cholesterolemic subjects after obtaining a written informed consent. Biochemical parameters like lipid profile, i.e., total cholesterol, low density lipoprotein cholesterol, triglycerides, high density lipoprotein cholesterol, very low-density lipoprotein cholesterol, kidney function test, Liver function test were assessed. Baseline information, their medical history and 24hr dietary recall was elicited from the subjects through a pretested schedule.
Results: The results have shown that the total cholesterol levels and its fractions along with triglycerides were significantly decreased, HDL-C increased significantly in the test groups from pre- supplementation to during-supplementation and were stable at post-supplementation period.
Conclusions: Our results indicated that the spice powder was rich in polyunsaturated fatty acid had a strong hypo cholesterolemic and hypotriglyceridemic effect.
Cardiovascular disease. Available at: https://www.who.int/news-room/fact-sheets/detail/ cardiovasculardiseases-(cvds)l. Accessed on 20 February 2023.
Rashed AN, Afifi FU, Disi AM. Simple evaluation of the wound healing activity of a crude extract of Portulacaoleracea L. (growing in Jordan) in Mus musculusJVI-1. J Ethnopharmacol. 2003;88:131-6.
Yazici I, Turkan I, Sekmen AH, Demiral T. Salinity tolerance of purslane (Portulacaoleracea L.) is achieved by enhanced antioxidative system, lower level of lipid peroxidation and proline accumulation. Env Exp Bot. 2007;61:49-57.
Lim YY, Quah EPL. Antioxidant properties of different cultivars of Portulacaoleracea. Food Chem. 2007;103:734-40.
Lee SJ, Shin JH, Kang MJ. Effects of Portulacaolerecea powder on lipid levels of rats fed a hypercholesterolemia inducing diet. Food Chem. 2011; 16:202-9.
Xu X, Yu L, Chen G. Determination of flavonoids in Portulacaoleracea L. by capillary electrophoresis with electrochemical detection. J Pharm Biomed Anal. 2006;41:493-9.
Simopoulos AP Kifer RR, Martin RE, Health Effects of Polyunsaturated Fatty Acids in Seafoods. Orlando, FL: Academic Press; 1986.
Simopoulos AP, Norman HA, Gillaspy JE. Purslane in human nutrition and its potential for world agriculture. World Rev Nutr Diet. 1995;77:47-74.
Oliveira I, Valentão P, Lopes R, Andrade PB, Bento A, Pereira JA. Phytochemical characterization and radical scavenging activity of Portulacaoleracea L. Microchem J. 2009;92:129-34.
Kim AR, Lee JJ, Lee YM, Jung HO, Lee MY. Cholesterol-lowering and anti- obesity effects of Polymniasonchifolia Poepp & Endl powder in rats fed a highfat-high cholesterol diet. J Korean Soc Food Sci Nutr. 2010;39:210-8.
Harnafi H, Aziz M, Amrani S. Sweet basil (Ocimumbasilicum L.) improves lipid metabolism in hypercholesterolemic rats. Food Chem. 2009;4:81-6.
Shin MK, Kim DH, Han SH. Effects of dried tea leaf powder of serum on lipid concentrations in rats fed high fat. Korean J Food Culture. 2003;18:226-34.
Won HR, Kim SH. Antihyperlipidemic effect of diet containing Portulacaoleracea L. ethanol extract in high fat diet-induced obese mice. J Korean Soc Food Sci Nutr. 2011;40:538-43.
Kim SH, Won HR. Effect of Portulacaoleracea Linne ethanol extract on lipid metabolism in rats fed high fat diet. J Life Nat Sci. 2010;17:51-60.
Kwon DK, Song YJ. Effect of swimming exercise and Portuluca oleraceae supplementation on metabolic parameters and immune function in fed a high-fat diet. Kor J Exer Nutr. 2010;14:23-9.
Allian C, Poon L, Richmond W, Fu P. Enzymatic determination of total serum cholesterol. Clin Chem. 1974;20:470-5.
Pisani T, Gebski CP, Leary ET. Accurate Dirrect determination of Low-density lipoprotein cholesterol using an immune separation agent and enzymatic cholesterol assays. Arch Pathol Lab Med. 1995;4: 1119-27.
Shepherd J, Packard CJ, Grundy SM. Effects of saturated and polyunsaturated fat diets on the chemical composition and metabolism of low density lipoproteins in man. J Lipid Res. 1990;21(1):91-9.
Sugiuchi H, Uji Y, Okabe H, Irie T. Direct measurement of high density lipoprotein cholesterol in serum with polyethylene Glycol-modified enzymes and sulphated alpha cyclodextrin. Clin Chem. 1995; 41(5):717-23.
Fabiny Dl, Ertingshahsen G. Automated reaction rate method for determination of serum creatinine with centrifichem. Clini Chem. 1971;17:696-700.
Fauwett JK, Scott JEA. Rapid and precise method for the determination of urea. J Clin Pathol. 1960;13:156-9.
Kageyama N. A direct calorimetric determination of uric acd in serum and urine with uricase catalase system. Clin Chim Acta. 1971;31:421-6.
Malloy HT and Evelyn KA. The determination of bilirubin with the photoelectric colorimeter. J Biol Chem. 1973;119:481-90.
Empfehlungen D, Gessels C. Standard Methode Zur B Esstimmug der Aktivitatderal kalischen Phosphatase. Klin Chem. 1972;10:191.
Berg MHU, Horder M, Rej R. Approved Recommendation on IFCC methods for te measurement of catalytic concentration of enzymes Part 3. J Cli Chem Clin Biochem. 1985;24;481-9.
Mensink RP, Katan MB. Effect of dietary fatty acids on serum lipids and lipoproteins. A meta-analysis of 27 trials. Arterioscler Thromb. 1992;12:911-9.
Yu S, Derr J, Etherton TD, Kris-Etherton PM. Plasma cholesterol-predictive equations demonstrate that stearic acid is neutral and monounsaturated fatty acids are hypocholesterolemic. Am J Clin Nutr. 1995;61: 1129-39.
Clarke R, Frost C, Collins R, Appleby P, Peto R. Dietary lipids and blood cholesterol: quantitative meta-analysis of metabolic ward studies. BMJ. 1997; 314:112-7.
Torsdottir I, Alpsten M, Holm G, Sandberg AS, T lli J. A small dose of soluble alginate-fiber affects postgrandialglycemia and gastric emptying in humans with diabetes. J Nutr. 1991;121:795-9.
Park BS Effect of dietary γ-linolenic acid on plasmalipid metabolism in rats. J Korean Oil Chemists Soc. 2002;19:181-8.
Lachhiramka P, Preety L, Sujay P. Cholesterol lowering property of garlic (Allium sativum) on patients with hypercholesterolemia. Int J Med Sci Pubic Health. 2016;5(11):2249-51.
Hoyos M, Guerrero R, Cano J, Olivan F, Fabiani A, Perganeda G, et al. Serum cholesterol and lipidperoxidation are decreased bymelatonin in diet induced hypercholesterolemicrats. J Pineal Res. 2000; 28:150-5.
Simopoulos A, Tan D, Manchester L, Reiter R. A plant source of omega -3 fattyacids and melatonin. J Pineal Res. 2005;39:331-2.
Rodriguez C, Mayo JC, Sainz RM. Regulation of antioxidant enzymes: A significant role for melatonin. J Pineal Res. 2004;36:1-9.
Feoli A, Roehrig C, Rotta L. Serum and liver lipids in rats and chiks fed with diets containing different oils. Basic Nutrit Investig. 2003;19:789-93.