Published: 2022-06-28

The importance of retesting borderline results in COVID-19 diagnostics

Edita M. Grego, Hristina A. Gospodinović, Ljiljana S. Pavlović, Verica S. Jovanović


The first case of COVID-19 in the Republic of Serbia was registered on 6 March 2020 based on data from the Ministry of Health of the Republic of Serbia.RT-PCR (real-time reverse transcription polymerase chain reaction) is the gold standard for detection for many infective agents. SARS-CoV-2 (human coronavirus 2019) comprises genes coding four basic structural pro­teins (E, M, N and S) and ORF1a and ORF1b genes cod­ing two polyproteins. These genes represent targets for virus detection and the platform for RT-PCR test design.


COVID-19, Real-time reverse transcription polymerase chain reaction, Ct value

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